MassIVE MSV000096314

Partial Public

GNPS - Nitrate in coastal waters: shifting the balance from carbon sink to carbon source

Description

Different salt marsh habitats, which are dominated by different plant species, will havedistinct metabolomic profiles that are driven by phyto-compounds released as plantexudates. However, within a given habitat, NO3 - will alter the metabolomic profile such thatthere will be a decrease in the most labile metabolites compared to reference marshes.Rates of decomposition depend on both the availability of a powerful electron acceptor anda supply of labile carbon that can be oxidized. In salt marshes that carbon is largelygenerated by primary production from halophytic plants, which have distinct zonationpatterns based on elevation above mean high water. The root exudates from these plants fuelmicrobial metabolism when there is a sufficient supply of electron acceptors present, but ourpreliminary metabolomic data suggest that different marsh habitats generate different plantexudates, which could largely explain why we see distinct microbial communitieswithin different marsh habitats. However, with our preliminary metabolomics datawe do not have enough samples to determine whether, within a given habitat, there is a shiftin the metabolic profile of the plant-microbe association that results from nutrientenrichment. The work (proposal:https://doi.org/10.46936/10.25585/60001192) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C5H98ZR36] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: salt marsh ; nitrogen enrichment ; DatasetType:Metabolomics

Contact

Principal Investigators:
(in alphabetical order)
Jennifer Bowen, Northeastern University, United States
Submitting User: bpbowen
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GNPS content goes here (MSV000096314 [task=545f68a5898744379819f2ab2a945a4e])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.