MassIVE MSV000090714

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GNPS - Relationship between thyroid hormones and associated metabolites and gene expression bioindicators in the serum of Rana [Lithobates] catesbeiana tadpoles and frogs during metamorphosis

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Description

Anuran metamorphosis is characterized by profound morphological changes including remodeling of tissues and organs. Here we investigate serum of Rana [Lithobates] catesbeiana (n=5-10) across seven distinct postembryonic stages beginning with premetamorphic tadpole (Gosner stage 31-33) and continuing through metamorphosis to a juvenile frog (Gosner stage 46). Using a sensitive nanoLC-Orbitrap system an untargeted analysis workflow was applied. Among 6,062 detected endogenous metabolites, 421 showed significant metamorphosis-dependent concentration dynamics. Among these potential bioindicators were several prostaglandins and other eicosanoids, some steroid hormones including testosterone, and several carnitines. These data provide a necessary context for interpreting developmental processes and potential molecular crosstalk mechanisms. Furthermore, this provides a solid foundation from which alternative bioindicators of metamorphosis may be chosen as feasible endpoints in future risk assessment of chemicals and their potency for causing developmental toxicity. [doi:10.25345/C5RJ4903J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: metabolomics ; Rana catesbeiana ; metamorphosis

Contact

Principal Investigators:
(in alphabetical order) 		Martin Hansen, Aarhus University, Denmark
Submitting User: Rikke

Publications

Poulsen R, Jackman SH, Hansen M, Helbing CC.
Relationship between serum thyroid hormones and their associated metabolites, and gene expression bioindicators in the back skin of Rana [Lithobates] catesbeiana tadpoles and frogs during metamorphosis.
Front Endocrinol (Lausanne). Epub 2023 Jan 18.

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