MassIVE MSV000082400

Complete Public PXD009886

Chapman_CCM_2018

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Description

This submission contains the mass spectrometry files for the manuscript by Chapman and colleagues that characterize KRI-1 in C. elegans by affinity purification experiments of KRI-1 fused to GFP. Controls used for this experiment include ACT-5 fused to GFP and N2 worms. This dataset consists of 18 raw MS files and associated with peak lists (mzML) and mass spectrometry results (mzid) in standard formats, acquired on a Thermo Orbitrap Elite Hybrid Ion Trap-Orbitrap operated in DDA mode. Samples were generated by Eric Chapman and affinity purification, mass spectrometric acquisition and analysis was performed by Christopher Go. Raw files are deposited here in MassIVE. Brent Derry is the corresponding author of the manuscript (brent.derry@sickkids.ca); Anne-Claude Gingras should be contacted for questions on this dataset (gingras@lunenfeld.ca). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Affinity purification ; Protein-protein interaction ; AP-MS ; KRI1_CCM1 ; Apoptosis ; Zinc Homeostasis ; Irradiation

Contact

Principal Investigators:
(in alphabetical order) 		Anne-Claude Gingras, LTRI, Canada
Submitting User: gingraslab

Publications

Chapman EM, Lant B, Ohashi Y, Yu B, Schertzberg M, Go C, Dogra D, Koskimäki J, Girard R, Li Y, Fraser AG, Awad IA, Abdelilah-Seyfried S, Gingras AC, Derry WB.
A conserved CCM complex promotes apoptosis non-autonomously by regulating zinc homeostasis.
Nat Commun. 2019 Apr 17;10(1):1791. Epub 2019 Apr 17.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.