MassIVE MSV000086888

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Proteomics of a microphysiological model of human trophoblast invasion during early embryo implantation

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Description

We have designed an implantation-on-a-chip device to model the invasion of specialized fetal extravillous trophoblasts (EVTs) from an implanting embryo into the maternal decidua. We profiled the proteome of the external factors secreted in the model, containing different cell population compositions: 1) Extravillous trophoblast monoculture - EVTs monoculture, 2) Endothelial cells monoculture - ECs monoculture, 3) EVTs + ECs coculture, and 4) EVTs, ECs, and decidualized stromal cells (DSCs) - triculture. The proteins were extracted using the MPLEx extraction method (Nakayasu et. al. PMCID: PMC5069757), digested with 1:50 (trypsin:protein, w:w) for 3 hr at 37 C. Solid Phase Extraction (SPE) was performed on the samples using 1 mL/50 mg columns from Phenomenex as previously described (PMCID: PMC7192326). Peptidic concentration was assayed using a BCA assay and 5 ul at 0.1 ug/ul were injected on the LC-MS/MS system. Data was searched with MaxQuant. [doi:10.25345/C5GJ58] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: organ-on-a-chip ; microphysiological systems ; implantation ; placenta

Contact

Principal Investigators:
(in alphabetical order) 		Dan Dongeun Huh, University of Pennsylvania, Philadelphia, United States
Geremy Clair, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Park JY, Mani S, Clair G, Olson HM, Paurus VL, Ansong CK, Blundell C, Young R, Kanter J, Gordon S, Yi AY, Mainigi M, Huh DD.
A microphysiological model of human trophoblast invasion during implantation.
Nat Commun. 2022 Mar 15;13(1):1252. Epub 2022 Mar 15.

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