Spectral count files from EphB2 BioID experiment found in "The endosomal sorting adaptor HD-PTP is required for ephrin-B:EphB signalling in motor axon guidance.". The parameters and protocol are described in detail in the methods section. In brief, we over expressed BirA* C-terminal tagged EphB2 in HEK 293 cells. We stimulated these cells with either media (no ligand), Fc or ephrin-B2-Fc in order to capture the protein landscape of ephrin-B2:EphB2 forward signalling.
BirA*-Flag-EGFP controls are VL_20150825_COT_05_AK.raw, VL_20150825_COT_06_AK.raw, VL_20150825_COT_07_AK.raw, VL_20150825_COT_08_AK.raw
Empty Vector controls are VL_20151116_COT_01_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_02_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_03_HEK293_pcDNA5EV_AK.raw, VL_20151116_COT_04_HEK293_pcDNA5EV_AK.raw
EphB2_Fc-BirA*-Flag samples are VL_20160623_COT_01_AK.raw, VL_20160718_COT_01_AK.raw, VL_20161116_COT_05_AK.raw, VL_20161116_COT_06_2_AK.raw
EphB2_NoLigand-BirA*-Flag samples are VL_20160630_COT_01_AK.raw, VL_20160630_COT_02_AK.raw, VL_20161116_COT_03_AK.raw, VL_20161116_COT_04_AK.raw
EphB2_EB2-BirA*-Flag samples are VL_20160623_COT_02_AK.raw, VL_20160718_COT_02_AK.raw, VL_20161118_COT_01_AK.raw, VL_20161118_COT_02_AK.raw
[doi:10.25345/C5S32F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: HD-PTP ; EphB2 ; ESCRT ; BioID ; Cell collapse ; Motor axon guidance
Principal Investigators: (in alphabetical order) |
Artur Kania, IRCM, Canada |
Submitting User: | halbagci |
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Owner | Reanalyses | |
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