In an attempt to identify proteins interacting with transcription factor E2F2, proteins extracted from HA-E2F2 overexpressing HEK293T cells were were subjected to immunoprecipitation (IP) with an anti-HA antibody or, as a negative control (NegCt), with an anti-T antibody. Immunoprecipitated proteins were eluted, digested with trypsin, and the resulting peptides were analyzed by LC-MS/MS. The whole procedure was repeated several times in an attempt to gauge reproducibility (nine replicates of the anti-HA IP and six replicates of the control IP).
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: E2F2 ; immunoprecipitation ; LC-MS/MS
Principal Investigators: (in alphabetical order) |
None Listed |
Submitting User: | ccms |
Osinalde N, Olea M, Mitxelena J, Aloria K, Rodriguez JA, Fullaondo A, Arizmendi JM, Zubiaga AM.
The nuclear protein ALY binds to and modulates the activity of transcription factor E2F2.
Mol. Cell Proteomics. 2013 May;12(5):1087-98. Epub 2013 Jan 7.
Number of Files: | |
Total Size: | |
Spectra: | |
Subscribers: | |
Owner | Reanalyses | |
---|---|---|
Experimental Design | ||
Conditions:
|
||
Biological Replicates:
|
||
Technical Replicates:
|
||
Identification Results | ||
Proteins (Human, Remapped):
|
||
Proteins (Reported):
|
||
Peptides:
|
||
Variant Peptides:
|
||
PSMs:
|
||
Quantification Results | ||
Differential Proteins:
|
||
Quantified Proteins:
|
||
Browse Dataset Files | Browse Results |
FTP Download Link (click to copy):
|