MassIVE MSV000094299

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GNPS - Comprehensive Untargeted Lipidomic Profiling of Third Generation Lentiviral Vectors and Packaging Cells: Raw LCMS Data

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Description

Untargeted high-resolution lipidomics data of HEK 293T packaging cells and lentiviral vectors (LV) in cell culture media. HEK 293T cells are treated with either vehicle control (DMSO), myriocin (positive control), expressing the VSV G protein or producing 3rd generation LV. LV are concentrated from HEK 293T cell culture media using ultracentrifugation. HEK 293T culture media from untransfected cells is also concentrated by ultracentrifugation and acquired as a lipidomics blank which is used to subtract the abundance of background lipids from the LV samples. Also included, is a diltution series of fetal bovine serum lipid extract (FBS) which is used to asses the linear dynamic range of the instrument. [doi:10.25345/C5S17T41V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Lipidomics ; HEK 293T ; Lentiviral Vectors

Contact

Principal Investigators:
(in alphabetical order) 		Jeffrey C. Smith, Carleton University, Canada
Submitting User: joshuaroberts
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Experimental Design
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Identification Results
    Proteins (Human, Remapped):
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Quantification Results
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GNPS content goes here (MSV000094299 [task=604c77163ea342a3a167fe74fe339464])
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.