Description
Proinlfammatory stimulation of murine macrophages elicits profound metabolic changes. iNOS-derived Nitric oxide (NO) is both necessary and sufficient for the repression of OXPHOS and major mitochondrial rewiring of carbon fluxes during M1 polarization. Since inflammation results in broad proteomics changes with oxidative modifications of specific targets, we aim to understand how this is managed in absence of this important endogenous gas and its derived reductive nitrogen species like nitroxyl (HNO).
We used proteomics to detail the mitochondrial proteome expression and status of cysteine modifications underlying proinflammatory stimulation and identified distinct targets of reversible and irreversible cysteine oxidations during this process in presence and absence of NOS2, RNS donors, and in exacerbating conditions of oxidative stress.
[doi:10.25345/C5JM23S00]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: macrophage, nitric oxide, nitroxyl, irreversible modification, free cysteines, inflammation, nitrosative stress
Contact
Principal Investigators:
(in alphabetical order)
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Daniel McVicar, National Cancer Institute (NCI), United States
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ronholes7059
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Distinct condition labels are counted across all files submitted in the "Metadata" category
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Originally identified proteins that were automatically
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SwissProt
human reference database.
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Number of distinct protein accessions reported across all analyses (original submission and
reanalyses) associated with this dataset.
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Number of distinct peptide sequences (including modified variants or peptidoforms) reported
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
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Number of distinct proteins found to be differentially abundant in at least one comparison
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A protein is differentially abundant if its change in abundance across conditions is found
to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated
with statistical tests for differential abundance.
Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
of Quantified Analytes" category having a "Protein" column in this dataset.
"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE.
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consist solely of processed peak lists suitable for reanalysis with most software.