MassIVE MSV000080302

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Identification of Phosphorylation Sites in RNA-binding Protein LIN28 in mESCs

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Description

LIN28 (also known as LIN28A), a highly conserved RNA-binding protein, has emerged as a central post-transcriptional regulator of cell fate through blockade of let-7 microRNA (miRNA) biogenesis and direct modulation of mRNA translation. To gain insight into how LIN28 is integrated with the pluripotency signaling network, we investigated the role of LIN28 phosphorylation. We employed a targeted phosphoproteomics strategy in mouse ESCs (mESCs) and were able to map four phosphosites, two of which, S184 and S200, were confidently assigned to specific serine residues. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: LIN28 ; mESCs ; phosphorylation

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Principal Investigators:
(in alphabetical order) 		George Q. Daley
Submitting User: ktsanov
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