Description
Hebert JD, Myers SA, Naba A, Abbruzzese G, Lamar J, Carr SA, Hynes RO.Metastasis causes most cancer-related deaths, and one poorly understood aspect of metastatic cancer is the adaptability of cells from a primary tumor to create new niches and survive in multiple, different secondary sites. We used quantitative mass spectrometry to analyze the extracellular matrix (ECM), a critical component of metastatic niches, in metastases to the brain, lungs, liver and bone marrow, all derived from parental MDA-MB-231 triple-negative breast cancer cells. We show that tumor and stromal cells cooperate in forming niches, with stromal cells producing predominantly core, structural ECM proteins and tumor cells producing a diverse array of ECM-associated proteins, including secreted factors and modulators of the matrix. Additionally, tumor and stromal cells together create distinct niches in each tissue, and we show that knocking down SERPINB1, a protein elevated in brain metastases, led to a reduction in brain metastasis, suggesting that some niche-specific ECM proteins may be involved in metastatic tropism.
[doi:10.25345/C5KD2P]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: extracellular matrix ; metastasis ; TMT10 ; Breast Cancer ; organotropism
Contact
Principal Investigators:
(in alphabetical order)
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Steven A. Carr, Broad Institute of MIT and Harvard, United States
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clauser
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis
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