MassIVE MSV000090379

Complete Public PXD036957

Unbiased proteomics profiling of pulmonary immune cells originating from irradiated lungs, non-irradiated lungs, or lungs from control mice

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Description

One murine lung was irradiated using 6Gyx5 X-ray exposure. Following irradiation, CD45+ (immune) were sorted from the ipsilateral (irradiated) and contralateral (non-irradiated) lung. Cells from non-irradiated controls were also collected. Proteins from the immune cells were extracted using the MPLEx method. Proteins were reduced with DTT, alkylated with Iodoacetamide, and digested using trypsin. 5 ul of 0.1 ug/ul of peptides were analyzed by LC-MS/MS on a Thermo Q Exactive Orbitrap instrument. [doi:10.25345/C5DZ0360T] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lung ; irradiation ; immune cells

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Principal Investigators:
(in alphabetical order) 		Geremy Clair, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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