MassIVE MSV000096827

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Hepatic p62, a major autophagic receptor in CYP4A ALD

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Description

In cell DSS crosslinking of HEK293 cells expressing His-tagged CYP4A11 and HA-tagged p62. Cells were treated with DSS, lysed, and enriched by Ni-NTA affinity chromatography. The eluate was separated by SDS-PAGE and the CYP-p62 crosslinked band identified by multi-channel western blot visualization. The crosslinked band was excised and in gel digested. Crosslinked peptides were analyzed on an Orbtrap Exploris 480 with a FAIMS source and running a 0.75 um x 50 cm c18 column with a 4-hour separation. The sample was injected twice using two different sets of compensation voltages: S20240820-04.raw: -45, -60, -70 V. S20240820-05: -40, -50, -65C. [doi:10.25345/C50P0X33C] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: In cell DSS crosslinking ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Maria Almira Correia, UCSF, USA
Submitting User: mtrnka

Publications

He L, Kwon D, Trnka MJ, Liu Y, Yang J, Li K, Totah RA, Johnson EF, Burlingame AL, Correia MA.
Liver CYP4A autophagic-lysosomal degradation (ALD): A major role for the autophagic receptor SQSTM1/p62 through an uncommon target interaction site.
bioRxiv. Epub 2024 Oct 14.

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