MassIVE MSV000087700

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Quantification on NOD1 ubiquitination sites with SspH2 co-overexpression

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Description

HEK293T cells were transiently transfected with NOD1-FLAG and HA-SspH2 WT/HA-SspH2 C580/empty vector. Following anti-FLAG automated immunoprecipitation and in-gel trypsin digestion, fractionated samples were injected onto LC-MS/MS. Quantitation was performed on diglycyl-lysine sites on NOD1, on identical peptides with highest intensity across samples. [doi:10.25345/C5XF9S] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Ubiquitination ; NOD1 ; SspH2 ; Diglycyl lysine ; Post-translational modifications

Contact

Principal Investigators:
(in alphabetical order) 		Amit Bhavsar, University of Alberta, Canada
Olivier Julien, University of Alberta, Canada
Submitting User: julienlab

Publications

Delyea CJ, Forster MD, Luo S, Dubrule BE, Julien O, Bhavsar AP.
The Salmonella Effector SspH2 Facilitates Spatially Selective Ubiquitination of NOD1 to Enhance Inflammatory Signaling.
Biochemistry. 2024 Sep 17;63(18):2266-2279. Epub 2024 Aug 27.

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