MassIVE MSV000094086

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Lacritin N-104 affinity purification of P. aeruginosa surface proteins

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Description

The precleared whole cell P. aeruginosa PA14 lysates underwent an overnight incubation with bead-immobilized N-104 peptide (or C-95 as a negative control peptide) under physiological conditions. Subsequent steps included washes and a gradual step-gradient elution ranging from 50 to 500 mM KCl. The eluate obtained at 500 mM KCl was then analyzed using tandem mass spectrometry to identify PA14 proteins that bind to N-104. [doi:10.25345/C5930P596] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lacritin ; N-104 ; P. aeruginosa ; PA14 ; YaiW

Contact

Principal Investigators:
(in alphabetical order) 		Gordon W. Laurie, Department of Cell Biology, University of Virginia, USA
Submitting User: anwaruddin
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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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