MassIVE MSV000100806

Complete Public PXD074350

Protection of teleomere1b safeguards the Arabidopsis genome by regulating ROS homeostasis

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Description

Proximity labeling proteomics samples generated using TurboID fused to AtPOT1b or AtPOT1a in Arabidopsis thaliana. Six-day-old seedlings expressing POT1b-TurboID or POT1a-TurboID and corresponding TurboID-only controls were grown on half-strength MS medium with or without sucrose. Seedlings were treated with 250 uM biotin for 1 hour, followed by streptavidin-based enrichment of biotinylated proteins and LC-MS/MS analysis. Each condition includes biological replicates. Raw files correspond to individual LC-MS/MS runs for each genotype and growth condition. [doi:10.25345/C5HD7P62W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Telomere, catalase, reactive oxygen species, oxidative stress response, 8-oxoG, genome integrity ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Dorothy E. Shippen, Texas A&M University, USA
Submitting User: alemoff
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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.