MassIVE MSV000097000

Partial Public PXD060377

Proteomic analysis of dried blood from the Sepsis Characterization in Kilimanjaro (SICK) study

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Description

Venous-EDTA whole blood was captured on 20 microliter Mitra devices at days 0,1,3,28 after hospital admission with Sepsis-2 criteria. Mitra tips were processed in Matrix tubes using deoxycholate-assisted in solution trypsin digestion. Approximately 1 mg of digests was enriched for N-glycopeptidesusing spin tips packed with Polyhydroxyethyl A followed by serial enrichment of phosphopeptides from the flow through using Ti-IMAC. LC-MS/MS of unenriched and phosphopeptide-enriched samples used microflow LC (30 samples-per-day) and Evosep LC (60 SPD), respectively, with data-dependent acquisition using an Orbitrap Astral MS. LC-MS/MS of N-glycopeptide-enriched samples used an Evosep LC (60 SPD) and either stepped-collision energy data-dependent acquisition (Exploris 480) or separate DDA and DIA with a 35% NCE using the Orbitrap Astral. Data analysis used Spectronaut and Glyco-Decipher. [doi:10.25345/C51R6NC5W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Mitra device ; whole blood ; N-glycoproteome ; phosphoproteome ; microflow ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Matthew W. Foster, Duke University, USA
Submitting User: mwfoster
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