MassIVE MSV000097000

Partial Public PXD060377

Proteomic analysis of dried blood from the Sepsis Characterization in Kilimanjaro (SICK) study

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Description

Venous-EDTA whole blood was captured on 20 microliter Mitra devices at days 0,1,3,28 after hospital admission with Sepsis-2 criteria. Mitra tips were processed in Matrix tubes using deoxycholate-assisted in solution trypsin digestion. Approximately 1 mg of digests was enriched for N-glycopeptidesusing spin tips packed with Polyhydroxyethyl A followed by serial enrichment of phosphopeptides from the flow through using Ti-IMAC. LC-MS/MS of unenriched and phosphopeptide-enriched samples used microflow LC (30 samples-per-day) and Evosep LC (60 SPD), respectively, with data-dependent acquisition using an Orbitrap Astral MS. LC-MS/MS of N-glycopeptide-enriched samples used an Evosep LC (60 SPD) and either stepped-collision energy data-dependent acquisition (Exploris 480) or separate DDA and DIA with a 35% NCE using the Orbitrap Astral. Data analysis used Spectronaut and Glyco-Decipher. [doi:10.25345/C51R6NC5W] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Mitra device ; whole blood ; N-glycoproteome ; phosphoproteome ; microflow ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Matthew W. Foster, Duke University, USA
Submitting User: mwfoster

Publications

Foster MW, McMahon TJ, Ngocho JS, Kipengele AH, Violette M, Chen Y, Madut DB, Plumb RS, Lan Wong A, Chen L, Lee GM, Sakasaka PA, Mmbaga BT, Crump JA, McClain MT, Woods CW, Maro VP, Rubach MP.
Mass Spectrometry-Based Quantification of Proteins and Post-Translational Modifications in Dried Blood: Longitudinal Sampling of Patients With Sepsis in Tanzania.
Proteomics. Epub 2025 Dec 3.

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