MassIVE MSV000090633

Complete Public PXD037886

Cilia-APEX proteomics profiling of Arl6-/- vs wiltype reveal UbK63 retrieval adaptors

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Description

Ascrobate peroxidase (APEX) was expressed as a fusion protein and directed to primary cilia of mouse inner medullary collecting duct (mIMCD-3) cells, where it was used for proximity labeling to biotinylate proteins, which are subsequently enriched by streptavidin chromatography and identified by mass spectrometry. Cilia-APEX proteomics profiling compared the cilia proteomes of wild-type and Arl6-/- cilia (generated by CRISPR/Cas9-mediated genome editing) by spectral counting. [doi:10.25345/C5707WT0V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proximity Labeling Proteomics ; cilia-APEX profiling

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Principal Investigators:
(in alphabetical order) 		Maxence V. Nachury, University of California San Francisco, USA
Submitting User: MickLab
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