HEK293T cells were transiently transfected with NOD1-FLAG, HA-SspH2 WT/HA-SspH2 C580/empty vector, and Myc-Ubiquitin. Following anti-FLAG automated immunoprecipitation and in-gel trypsin digestion, fractionated samples were injected onto LC-MS/MS. Quantitation was performed on diglycyl-lysine sites on NOD1, on identical peptides with highest intensity across samples.
[doi:10.25345/C5TN8J]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: NOD1 ; SspH2 ; Ubiquitination ; Diglycyl lysine ; Post-translational modifications
Principal Investigators: (in alphabetical order) |
Amit Bhavsar, University of Alberta, Canada Olivier Julien, University of Alberta, Canada |
Submitting User: | julienlab |
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Owner | Reanalyses | |
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