MassIVE MSV000082403

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Reproducibility of a WGA-Jacalin based affinity enrichment of O-glycopeptides from human serum tryptic digest

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Description

Human serum tryptic digest was enriched on a WGA-POROS affinity column collecting two glycopeptide fractions. The fractions were treated with neuraminidase and O-glycopeptides were isolated by a subsequent Jacalin affinity chromatography. Peptide mixtures were analyzed by LC/MS-MS applying HCD fragment ion dependent ETD analysis using an Orbitrap Elite mass spectrometer. Technical reproducibility was assessed using 3 parallel samples. [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: reproducibility, lectin affinity chromatography, O-glycosylation, HCD, ETD, Pinnacle

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Principal Investigators:
(in alphabetical order) 		Zsuzsanna Darula, Biological Research Centre, Hungary
Submitting User: protlabszeged
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