A peptidomic analysis of plasma from patients with well characterised pancreatic neuroendocrine tumours (PNET) was performed. A nano LC-MS analysis identified a number of peptides from the glucagon gene, which were idenfiried in a previous case study using multiple immunoassays. Peptides to other proteins involved in peptide secretion were also identified. Plasma from a second glucagonoma patient was analysed using a high flow approach, and this identified similar peptides to the nano analysis. In order to demonstrate the potential clinical application of LC-MS to characterising neuroendocrine tumours, a large cohort of plasma samples were analysed to demonstrate the ability of this approach to identify glucagonoma patients, and differentiate from a single insulinoma patient
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Pancreatic neuroendocrine tumour ; plasma peptidomics ; glucagonoma
Principal Investigators: (in alphabetical order) |
Professor Fiona Gribble, Metabolic Research Laboratories, University of Cambridge, N/A |
Submitting User: | ccms |
Kay RG, Challis BG, Casey RT, Roberts GP, Meek CL, Reimann F, Gribble FM.
Peptidomic analysis of endogenous plasma peptides from patients with pancreatic neuroendocrine tumours.
Rapid Commun. Mass Spectrom. 2018 Aug 30;32(16):1414-1424.
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