MassIVE MSV000084800

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Ubiquitin Proteomics Analysis of Systemic Sclerosis Lung Fibroblasts with or without KLHL42 knockdown

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Description

Systemic scleroderma (SSc) is an autoimmune disease which results in fibrotic production in the lung. Resultant SSC-pulmonary fibrosis is the main cause of mortality among SSc patients. From high throughput RNAi screening, we uncovered the ubiquitin E3 ligase KLHL42 as a potential pro-fibrotic mediator of TGFb-dependent fibrotic signaling in primary SSc lung fibroblasts. In this analysis, we sought to uncover putative substrates for KLHL42 by comparing SSc lung fibroblasts with control or KLHL42 siRNA prior to TGFb-treatment, lysis, and TUBE precipitation. The resultant pull-down was analyzed with LC-MS/MS. [doi:10.25345/C5TX08] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: ubiquitin ; Klhl42 ; Systemic Sclerosis

Contact

Principal Investigators:
(in alphabetical order) 		Bill Chen, University of Pittsburgh, United States
Submitting User: tblear

Publications

Lear TB, Lockwood KC, Larsen M, Tuncer F, Kennerdell JR, Morse C, Valenzi E, Tabib T, Jurczak MJ, Kass DJ, Evankovich JW, Finkel T, Lafyatis R, Liu Y, Chen BB.
Kelch-like protein 42 is a profibrotic ubiquitin E3 ligase involved in systemic sclerosis.
J Biol Chem. 2020 Mar 27;295(13):4171-4180. Epub 2020 Feb 17.

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