MassIVE MSV000090045

Partial Public PXD035651

Pull-Down of UCHL1 from living cells with small molecule probes GK13S, GK13R, and GK16S (1 uM)

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Description

UCHL1 was enriched from HEK293 cells (Homo Sapiens) using a probe (PullDown via NeutrAvidin beads). Protein enrichment as a function of substance addition was studied in comparison to a DMSO control. [doi:10.25345/C5N29PB3Q] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: bottom-up proteomics ; interaction analysis

Contact

Principal Investigators:
(in alphabetical order) 		Petra Janning, MPI of Molecular Physiology, Germany
Submitting User: janning

Publications

Christian Grethe, Mirko Schmidt, Gian-Marvin Kipka, Rachel O'Dea, Kai Gallant, Petra Janning, Malte Gersch.
Structural basis for specific inhibition of the deubiquitinase UCHL1.
Nat Commun. 2022 Oct 10;13(1):5950. doi: 10.1038/s41467-022-33559-4.

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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.