MassIVE MSV000096580

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Spaceflight alters protein levels and gene expression associated with cell survival and metabolic characteristics in human cardiac spheroids

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Description

Cardiomyocytes derived from human induced pluripotent stem cells (hiPSC-CMs) possess tremendous advantage for cardiac regeneration. However, cell survival is challenging upon cell transplantation. Since microgravity can profoundly affect cellular properties, we investigated the effect of spaceflight on hiPSC-CMs. Cardiac spheroids derived from hiPSCs were transported to the International Space Station (ISS) via the SpaceX Crew-8 mission and cultured under space microgravity for 8 days. Beating cardiac spheroids were observed on the ISS and upon successful experimentation by the astronauts in space, the live cultures were returned to Earth. These cells had normal displacement (an indicator of contraction) and Ca2+ transient parameters in 3D live cell imaging. Proteomics analysis revealed that spaceflight upregulated many proteins involved in metabolism (n=90), cellular component of mitochondrion (n=62) and regulation of proliferation (n=10). Specific metabolic pathways enriched by spaceflight included glutathione metabolism, biosynthesis of amino acids, and pyruvate metabolism. In addition, spaceflight upregulated proteins in cellular stress response, cell survival, and the AMPK signaling pathway, a master regulator of metabolism. [doi:10.25345/C5NP1WW60] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Microgravity ; Human induced pluripotent stem cells ; hiPSCs ; Proteomics ; Metabolic pathways ; AMPK signaling pathway ; Cell survival ; Cellular stress response ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Chunhui Xu, Department of Pediatrics, Emory University School of Medicine and Children's Healthcare of Atlanta; Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, USA
Submitting User: Zeyu
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