MassIVE MSV000100012

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Aerobic catabolism of isobutylene by Mycolicibacterium sp. ELW1 requires plasmid-borne ibc genes

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Description

Shotgun proteomic analysis was performed on batch cultures of strain Mycolicibacterium sp. ELW1 grown on IB (10%, v/v, gas phase) or fructose (final concentration ~10 mM) in MSM media. Cells grown to ~mid log phase in a dark shaking incubator (150 rpm; at 30C). Bacterial pellets (sample sizes IB-grown: n=3 and fructose-grown: n=3) were submitted to the UC Davis Proteomics Core Facility for protein extraction, digestion, and liquid chromatography tandem mass spectrometry (LC-MS/MS) using a timsTOF HT/EvoSep. Raw data files along with the differential expression analysis are provided. The results (.sne file) can be found at https://doi.org/10.5281/zenodo.16985746. [doi:10.25345/C5TT4G645] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Mycolicibacterium sp. ELW1 ; Isobutylene ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Michael Hyman, North Carolina State University, United States
Submitting User: jbjoyce
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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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