30 ug of ginger ethanol extract (1% DMSO) was spiked into an E. coli lysate containing 1 to 10 uM of a His-tagged NR LBD fusion protein. Following incubation and Nickel affinity purification, the samples were analyzed by untargeted LC-MS to identify masses enriched by the NR pulldown. Parallel pull-downs were conducted using an NR LBD fusion protein without the addition of the ginger extract as control.
[doi:10.25345/C5513V650]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: ginger, human nuclear receptor
Principal Investigators: (in alphabetical order) |
Henry Krause, University of Toronto, Canada |
Submitting User: | ljb5021 |
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Owner | Reanalyses | |
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