MassIVE MSV000089658

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The identification of binding partners of mitochondrial microprotein, SHLP2

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Description

For SHLP2_ carboxyLinkTM kit set, we used carboxyLinkTM kit to create a SHLP2 peptide column and a scrambled peptide column as a control to identify direct SHLP2 binding proteins. The mitochondria protein extract were used to detect direct binding partners of SHLP2. For SHLP2_APEX set, APEX2 proximity labeling method is used to identify the binding proteins of SHLP2, a mitochondrial microprotein. Plasmid harboring SHLP2-APEX fusion proteins (SHLP2-APEX-myc) were transfected into cells The biotinylated proteins were enriched by streptavidin beads pulldown. Two controls were used the APEX control (Flag-APEX-myc) and MA control (MTS (mitochondrial targeting sequence)-APEX-myc). [doi:10.25345/C5WH2DJ71] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: SHLP2, mitochondrial microprotein, APEX proximity labeling

Contact

Principal Investigators:
(in alphabetical order) 		Pinchas Cohen, USC, United States
Submitting User: sujkim
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