MassIVE MSV000096102

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Ion Mobility-Assisted Free Radical Initiated Peptide Sequencing v2

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Description

Free radical-initiated peptide sequencing (FRIPS) is a tandem mass spectrometry technique (MS/MS) that enables radical-based dissociation on instruments only capable of collisional activation. In FRIPS, peptides are chemically-derivatized with a compound that undergoes homolytic cleavage and generates radicals upon collisional activation. These radicals then propagate through the peptide backbone enabling the sequencing of peptide ions. This MS/MS technique has shown promise in sequencing post-translationally modified peptides, but it is typically performed in an MS3 workflow and single-step MS/MS approaches result in the generation of both collisional- and radical-driven dissociation products and highly complex spectra. Recently, our group developed a method to dissociate peptide ions prior to ion mobility analysis within a trapped-ion mobility spectrometry (TIMS) device. In this work, we examine if this CIDtims technique is capable of initiating the homolytic cleavage of the FRIPS precursor. We then examine if the resultant ion mobility separation results in additional assignments of product ions and improved sequence coverage. We demonstrate that activation within the TIMS device does indeed promote robust radical initiation and fragmentation and that the generated product ions are mobility separated enabling facile assignment and increased sequence coverage. [doi:10.25345/C5BN9XF31] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: mass spectrometry ; ion mobility ; DatasetType:Other (tandem mass spectrometry)

Contact

Principal Investigators:
(in alphabetical order) 		Nicholas Borotto, University of Nevada, United States
Submitting User: nborotto
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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