MassIVE MSV000083503

Partial Public PXD012918

Analysis of sorted human peripheral blood mononuclear cells by HUNTER (High-efficiency Undecanal-based N Termini EnRichment)

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Description

Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samples. Identification of distinct N-terminal profiles in human peripheral blood mononuclear sorted cells (B-cells; NK; NKT; monocytes) as one of the applications of HUNTER. [doi:10.25345/C5QK8X] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HUNTER ; N termini ; proteolytic proteoforms ; proteomics ; mononuclear cells ; B-cells ; NK ; NKT ; monocytes

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Philipp Lange, The University of British Columbia, Canada
Dr. Pitter F. Huesgen, University of Cologne, Germany
Submitting User: LangeLab

Publications

Weng SHS, Demir F, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF, Lange PF.
Sensitive determination of proteolytic proteoforms in limited microscale proteome samples.
Mol. Cell Proteomics. Epub 2019 Aug 30.

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