MassIVE MSV000096927

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Sucrose utilisation strategies in phototrophic purple bacteria: paving the way to sugar-rich by-product valorisation

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Description

The circular bioeconomy has become a crucial strategy for sustainable development, especially by upcycling organic-rich by-products from various industrial processes. Purple non-sulphur bacteria (PNSB) have emerged as excellent candidates in this field, demonstrating exceptional metabolic versatility. While the growth of PNSB on sugar-rich streams has been extensively explored, the sugar assimilation metabolism remains poorly understood. Here, we explore the metabolic mechanisms of sucrose utilisation in two phototrophic purple non-sulphur bacteria (PNSB), Rhodospirillum rubrum and Rhodobacter capsulatus. Our findings demonstrate distinct carbohydrate hydrolysis and assimilation capacities, as well as the use of different redox strategies for each species. Moreover, Rhodospirillum rubrum could only grow on sucrose when co-cultivated with Rhodobacter capsulatus. This trophic link between Rhodospirillum rubrum and Rhodobacter capsulatus in sucrose containing co-culture was characterised and resulted in significantly enhanced productivity compared to pure cultures. Finally, we demonstrate that the synergy observed between Rhodospirillum rubrum and Rhodobacter capsulatus can be successfully scaled up in a photobioreactor system. Our study highlights how fundamental knowledge of the metabolism, trophic link and general microbial ecology concept might be useful for the development of biobased resource recovery strategies. [doi:10.25345/C5G737G50] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Rhodospirillum rubrum ; sugar ; Proteomics ; by-product valorisation ; DatasetType:Other (Proteomics)

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Principal Investigators:
(in alphabetical order) 		Manon Gilson, University of Mons, Belgique
Submitting User: Manon_Gilson
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