MassIVE MSV000095232

Partial Public

GNPS - Community-wide interactions sustain life in geothermal spring habitats

Description

We investigated an alga-dominated geothermal spring community in Yellowstone National Park (YNP), USA to determine how the biota cope with abiotic stressors. Microbes showed a community level response to toxic metal resistance and energy cycling that spans the three domains of life. Arsenic detoxification is accomplished via complementary expression of genes by different lineages. Photosynthetic primary production is dominated by the obligate photoautotrophic alga Cyanidioschyzon, with the mixotroph, Galdieria, largely relegated to nighttime heterotrophy. Many key functions, including the cell cycle, are strongly regulated by diurnal fluctuations in light and nutrients. These results demonstrate that biotic interactions are highly structured and constrained in extreme habitats. We suggest this was also the case on the early Earth when geothermal springs were cradles of microbial life. The work (proposal:https://doi.org/10.46936/10.25585/60000481) conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE Office of Science User Facility, is supported by the Office of Science of the U.S. Department of Energy operated under Contract No. DE-AC02-05CH11231. [doi:10.25345/C5794157B] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: geothermal spring ; arsenic detoxification ; algae ; Cyanidioschyzon ; Galdieria

Contact

Principal Investigators:
(in alphabetical order)
Debashish Bhattacharya, Rutgers University, United States
Submitting User: bpbowen
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GNPS content goes here (MSV000095232 [task=7b95c3e1865444ebbc60d1316517d7f0])
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Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.