MassIVE MSV000090591

Partial Public

Phosphorylation of pyruvate dehydrogenase inversely associates with neuronal activity

Description

For decades, the expression of immediate early genes (IEGs) such as FOS has been the most widely used molecular marker representing neuronal activation. However, to date, there is no equivalent surrogate available for the decrease of neuronal activity. Here, we developed an optogenetic-based biochemical screen in which population neural activities can be controlled by light with single action potential precision, followed by unbiased phosphoproteomic profiling. We identified that the phosphorylation of pyruvate dehydrogenase (pPDH) inversely correlated with the intensity of action potential firing in primary neurons. In in vivo mouse models, monoclonal antibody-based pPDH immunostaining detected activity decreases across the brain induced by a wide range of factors including general anesthesia, chemogenetic inhibition, sensory experiences, and natural behaviors. Thus, as an inverse activity marker (IAM) in vivo, pPDH can be used together with IEGs or other cell-type markers to profile and identify bi-directional neural dynamics induced by experiences or behaviors. [doi:10.25345/C5NC5SH67] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Neuron Activity ; neuronal activity ; immediate early genes ; inverse activity marker

Contact

Principal Investigators:
(in alphabetical order)
Li Ye, Scripps Research, United States Of America
Submitting User: titusj
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