MassIVE MSV000087990

Partial Public PXD027956

Peroxynitrite in tumor microenvironment change the profile of peptide epitopes on tumor cells allowing their escape from cancer immunotherapy

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Description

SILAC quantitation of MHC I peptides in mouse and human samples using LC-MS/MS. GabrilovichD-18-L005 is a representative experiment for PNT treatment of murine EG.7 with PNT and cultured afterwards for the specified amount of time (specified in the file name, in 2 replicates for 30 min time-point). GabrilovichD-17-L134 (93-2017UACC60min) is a PNT treatment of human melanoma UACC903 cells and cultured afterwards for 30 mins. GabrilovicD-20-L066 (B16mix1-HF and mix2) is an experiment with overexpression of iNOS in B16 cells (2 replicates). GabrilovichD_20-L070 is bortezomib treatment of EG7 cells for 50 min (mix1), 120 min (mix2), 300 min (mix4). Treated cells were "heavy" labeled, control cells were "light" labeled. [doi:10.25345/C53N9D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Peroxynitrite ; Tumor microenvironment ; SILAC ; MHC I peptide

Contact

Principal Investigators:
(in alphabetical order) 		Dmitry Gabrilovich, AstraZeneca, United States
Submitting User: tangh
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Experimental Design
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.