MassIVE MSV000087733

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Integrated transcriptome and proteome profiling reveals a set of up-regulated genes/proteins related to low density neutrophils and impairment in transcription and translation processes in clinical sepsis

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Description

Sepsis is a global health emergency, which is known to be caused by various sources of infection that lead to changes in gene expression, protein coding, and metabolism. Advancements in omics technologies have provided valuable tools to unravel the mechanisms involved in the pathogenesis of this disease. Through integration of transcriptome and proteome profiling, we identified 170 co differentially expressed genes/proteins. Among these, 122 genes/proteins displayed the same expression trend. Protein protein interaction network analyses revealed two densely connected regions, which majorly included down regulated genes/proteins that were related to the transcription of RNA, translation of proteins, and mitochondrial translation. Additionally, we identified one module comprising of up regulated genes/proteins, which were mainly related to low density neutrophils (LDNs). IPA analysis revealed enrichment of canonical pathways and diseases or functions annotations, wherein pathways related to lymphocyte functions with decreased status, and defense processes that were predicted to be strongly increased. These findings showed a reprioritization of biological functions in response to sepsis that involved a transcriptional and translational shutdown of genes/proteins, with exception of a set of genes related to LDNs and host defense system. [doi:10.25345/C5NV7P] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: LDNs, Omics, Transcriptional Shutdown, Translation Shutdown, Sepsis

Contact

Principal Investigators:
(in alphabetical order) 		Alexandre Keiji Tashima, Escola Paulista de Medicina/UNIFESP, Brasil
Reinaldo Salomao, Escola Paulista de Medicina/UNIFESP, Brazil
Submitting User: aktashima

Publications

Leite GGF, Ferreira BL, Tashima AK, Nishiduka ES, Cunha-Neto E, Brunialti MKC, Assuncao M, Azevedo LCP, Freitas F, van der Poll T, Scicluna BP and Salomão R.
Combined Transcriptome and Proteome Leukocyte’s Profiling Reveals Up-Regulated Module of Genes/Proteins Related to Low Density Neutrophils and Impaired Transcription and Translation Processes in Clinical Sepsis.
Front. Immunol. 2021; 12:744799. doi: 10.3389/fimmu.2021.744799.

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