MassIVE MSV000097015

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GNPS - Extended coverage of human serum glycosphingolipidome by 4D-RP-LC TIMS-PASEF unravels association with Parkinson's disease

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Description

Glycosphingolipids (GSLs) are important targets in immune, infectious, lysosomal storage diseases, cancer, and neurodegenerative diseases. Circulatory GSL profiling in clinical samples is restricted by the lack of mid- and high-throughput analytical methods and deep coverage of long-chain sialylated glycosphingolipidome. We present a 4-dimensional (4D)-glycosphingolipidomics platform for routine glycosphingolipidome profiling encompassing: extraction and fractionation of sialylated GSLs with 3 to 15 monosaccharides, neutral GSLs and sulfatides; µL-flow reversed-phase LC-TIMS-PASEF MS analysis; semi-quantification strategy adapted for fractionated glycosphingolipidome, and referential CCS, RT, and m/z values for GSL annotation. 4D-glycosphingolipidomics of human serum reveals a high structural heterogeneity, amounting to 376 GSLs: 159 GSLs of ganglio- and neolacto-series, 145 neutral GSLs and 72 sulfatides. Here we demonstrate the platform’s utility for clinical profiling of Parkinson’s disease (PD) sera. 41 neolacto- and ganglio-species discriminate PD patients from controls and 14 GSLs differentiate sex subgroups, laying the foundation for further functional GSL studies with PD. [doi:10.25345/C53J39C93] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: lipidomics ; glycosphingolipids ; TIMS PASEF ; ion mobility mass spectrometry ; DatasetType:Other (Lipidomics)

Contact

Principal Investigators:
(in alphabetical order) 		Laura Bindila, Clinical Lipidomics Unit, Institute of Physiological Chemistry, University Medical Center Mainz, Germany
Submitting User: huovo
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GNPS content goes here (MSV000097015 [task=7ea04cbbd9b04840a3d3dcb9c5c1b846])
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