This experiment analyzes the global impact of Atg5-/- mutation on steady-state protein levels by a standard SILAC experiment. WT+vector cells were grown in isotopically labeled media for multiple passages in order to fully label the proteome. Unlabeled Atg5-/- cells and labeled WT+vector cells were grown to confluency and protein extracts from quiescent cultures were combined at a 1:1 ratio and analyzed by LC-MS/MS. In this experiment, ratios of labeled to unlabeled spectra report on changes in steady-state expression levels of proteins induced by impairment of autophagy. Table of files Cells Fractionation Raw Data Filenames Wildtype & Atg5-/- Yes SILAC_(1-8).raw (8 fractions)
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: human primary fibroblasts ; Atg5 ; steady state protein level
Principal Investigators: (in alphabetical order) |
Sina Ghaemmaghami, Department of Biology, University of Rochester, NY, USA, N/A |
Submitting User: | ccms |
Zhang T, Shen S, Qu J, Ghaemmaghami S.
Global Analysis of Cellular Protein Flux Quantifies the Selectivity of Basal Autophagy.
Cell Rep. 2016 Mar 15;14(10):2426-39. Epub 2016 Mar 3.
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