MassIVE MSV000090917

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Bottom-up Proteomics Analysis for Adduction of the Broad Spectrum Herbicide Atrazine to Histone

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Description

Whole histones of calf thymus or human histone H3.3 was incubated with atrazine. After solvent-based protein precipitation, the protein was digested by trypsin/Glu-C and the resulting peptides were analyzed by LC-MS/MS. The resulting tryptic/Glu-C peptide of DTNLCAIHAK from calf thymus or human H3.3 was identified with an accurate mass shift of +179.117 Da in atrazine incubated samples. It is deduced that a chemical group with elemental composition of C8H13N5 (179.1171 Da) from atrazine adducted with calf thymus or human histone H3.3. It was confirmed by MS/MS analysis that the adduction position was at its cysteine110 residue. [doi:10.25345/C54Q7QV4G] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: histone ; atrazine ; adduct ; LC-MS/MS

Contact

Principal Investigators:
(in alphabetical order) 		Robert J. Letcher, Environment and Climate Change Canada, Canada
Shaogang Chu, Environment and Climate Change Canada, Canada
Submitting User: ChuSG
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