MassIVE MSV000100796

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Co-precipitated proteins with Cfap298 antibody from wilde type mouse testes

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Description

Immunoprecipitation (IP) was performed using testis lysates from wild type mice. Briefly, lysates were adjusted to a protein concentration of 60 mg/mL, and 1000 micro litter (total 60 mg protein) was used for each reaction.For IP, rabbit normal IgG (control) or rabbit anti-CFAP298 antibody was added at 60 micro gram per sample and incubated with rotation for 2 h to allow antibody binding. Subsequently, DynabeadsTM Protein G were added, and samples were further incubated for an hour with gentle rotation. Afterward, the beads were washed thrice with lysis buffer and once with PBS to remove nonspecific proteins. Bound proteins were eluted using Tricine Sample Buffer to a final volume of 70 micro litter. Of the elute the 62 micro litter was submitted for proteomic analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) at a commercial service provider. [doi:10.25345/C5ST7F98V] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Cfap298 ; mouse testis ; IP ; co-precipitated ; DatasetType:Proteomics

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Principal Investigators:
(in alphabetical order) 		Heon Yung Gee, Yonsei University, South Korea
Submitting User: subyujin
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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