MassIVE MSV000088429

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Novel Brutons Tyrosine Kinase (BTK) substrates for time-resolved luminescence assays

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Description

BTK substrate profile determined by treating digest KG-1 cell lysate with alkaline phosphatase followed by recombinant BTK. Samples were enriched with PolyMac phosphoenrichment kits prior to analysis. We have organized our uploaded files into three main folders: 1. BTK KALIP MS raw files: raw mass spectrometer files in .RAW format. 2. BTK KALIP PEAKS analysis: project files from data analysis using PEAKS Studio X pro. These files are formatted as a PEAKS projects and can be opened in PEAKS software. 3. BTK PEAKS output files: exported files from the PEAKS Studio X pro analysis, including protein-peptides.csv files for each replicate. [doi:10.25345/C58P2N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: BTK ; phosphoproteomics

Contact

Principal Investigators:
(in alphabetical order) 		Laurie Parker, University of Minnesota, USA
Submitting User: nwid

Publications

Widstrom NE, Perez M, Pratt ED, Heier JL, Blankenhorn JF, Breidenbach L, Peterson H, Parker LL.
Novel Bruton's Tyrosine Kinase (BTK) Substrates for Time-Resolved Luminescence Assays.
ACS Chem Biol. 2022 Jun 17;17(6):1328-1333. Epub 2022 Jun 2.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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