HeLa cells were with Tunicamycin or Thapsigargin for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides were analyzed on a Thermofisher scientific Q Exactive HFX.
[doi:10.25345/C51631]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Proteomics ; Label-free ; HeLa ; ER stress ; Proteasome
Principal Investigators: (in alphabetical order) |
Bertrand Fabre, Technion, Israel |
Submitting User: | bertrandfabre31 |
Fabre B, Livneh I, Ziv T, Ciechanover A.
Identification of proteins regulated by the proteasome following induction of endoplasmic reticulum stress.
Biochem. Biophys. Res. Commun. 2019 Sep 17;517(2):188-192. Epub 2019 Jul 18.
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