MassIVE MSV000083736

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Regulation of proteome by the proteasome upon ER stress

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Description

HeLa cells were with Tunicamycin or Thapsigargin for 8 hours in the presence or absence of MG132 or untreated. Cells were lysed by addition of HEPES 50 mM pH7.9 with 5% SDS. Proteins were precipitated with methanol/chloroforme and the proteins were resolubilized in 8 M Urea and digested with trypsin. The peptides were analyzed on a Thermofisher scientific Q Exactive HFX. [doi:10.25345/C51631] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Proteomics ; Label-free ; HeLa ; ER stress ; Proteasome

Contact

Principal Investigators:
(in alphabetical order) 		Bertrand Fabre, Technion, Israel
Submitting User: bertrandfabre31

Publications

Fabre B, Livneh I, Ziv T, Ciechanover A.
Identification of proteins regulated by the proteasome following induction of endoplasmic reticulum stress.
Biochem. Biophys. Res. Commun. 2019 Sep 17;517(2):188-192. Epub 2019 Jul 18.

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