MassIVE MSV000086156

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Ubiquitylome analysis of Arabidopsis roots.

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Description

Ubiquitylome analysis of Arabidopsis roots. 10 day old Arabidopsis roots were treated with mock, DMSO, or bortezomib for 3 hours. Root tissue was processed using the phenol-FASP method described in https://doi.org/10.1002/pmic.201800220. Ubiquitin remnant peptides were enriched using an anti-diglycine lysine antibody from PTM-biolabs (PTM-1104). non-enriched as well as di-gly enriched peptides were labeled with TMT10 or TMT16 reagents. Both label-free and TMT labeled peptides were analyzed by LC-MS. [doi:10.25345/C5F17J] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Ubiquitylome ; Degradation ; TMT

Contact

Principal Investigators:
(in alphabetical order) 		Justin Walley, Iowa State University, US
Submitting User: jwalley

Publications

Wang P, Clark NM, Nolan TM, Song G, Bartz PM, Liao CY, Montes-Serey C, Katz E, Polko JK, Kieber JJ, Kliebenstein DJ, Bassham DC, Walley JW, Yin Y, Guo H.
Integrated omics reveal novel functions and underlying mechanisms of the receptor kinase FERONIA in Arabidopsis thaliana.
Plant Cell. 2022 Jul 4;34(7):2594-2614.

Song G, Montes C, Olatunji D, Malik S, Ji C, Clark NM, Pu Y, Kelley DR, Walley JW.
Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis.
Plant Cell. Epub 2024 Nov 21.

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