Protein N-termini reveal fundamental regulatory mechanisms and their perturbation in disease. We present a robust, sensitive, scalable and automatable method called High-efficiency Undecanal-based N Termini EnRichment (HUNTER) for system-wide identification of thousands of N-termini from minute samples. Comparison of enrichment performance between the HUNTER protocol and well-established TAILS protocol was based on HeLa cell lysates.
[doi:10.25345/C5RW8B]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: HUNTER ; N termini ; proteolytic proteoforms ; proteomics ; TAILS
Principal Investigators: (in alphabetical order) |
Dr. Philipp Lange, The University of British Columbia, Canada Dr. Pitter F. Huesgen, University of Cologne, Germany |
Submitting User: | LangeLab |
Weng SHS, Demir F, Ergin EK, Dirnberger S, Uzozie A, Tuscher D, Nierves L, Tsui J, Huesgen PF, Lange PF.
Sensitive determination of proteolytic proteoforms in limited microscale proteome samples.
Mol. Cell Proteomics. Epub 2019 Aug 30.
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