MassIVE MSV000094678

Partial Public PXD051994

Single cell proteomics of an HDAC inhibitor by label free proteomics

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Description

NCI-H-358 cancer cells were treated with the HDAC inhibitor mocetinostat for 24 hours. Single cells were isolated by FACs and prepared in a single well prior to diaPASEF analysis on a TIMSTOF SCP. A 40cm column was used for all analysis using a 200nL/min active gradient of 180 minutes. Library runs were created by pooling 16 single control and treated cells and running on the same gradient (separately) and processed with SpectroNaut 18. [doi:10.25345/C5T14V132] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Single cell proteomics ; Mocetinostat ; Histone deacetylase inhibitor ; Single cell epigenetics ; Single cell epiproteomics

Contact

Principal Investigators:
(in alphabetical order) 		Benjamin C Orsburn, Johns Hopkins, USA
Submitting User: ben_orsburn
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