MassIVE MSV000095579

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In Vitro Exposures of A549, J774A.1 Cells to SiO2, TiO2 Nanoforms and Related Cellular and Molecular Level Effects: Application of Proteomics

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Description

There is emerging interest in using proteomic data for environmental health-risk assessments. Meanwhile, due to attractive physicochemical properties, production and use of engineered nanomaterials (ENMs) are expanding with potential for exposure, thus necessitating toxicity information on these materials for human health risk analysis, where proteomic data can be informative. Here, cells (A549 human lung epithelial, J774 mouse monocyte/macrophage) were exposed to ENMs (nanoforms of SiO2, TiO2) of different sizes, surface chemistries (dose:0-100 ug/cm2, 24 h) for in vitro toxicity data. Cellular cytotoxicity (CTB, ATP, LDH), oxidative stress and proteomic analysis (MS-, antibody-based) were conducted post-nanoparticle (NP) exposures to determine cytotoxic potencies and identify molecular-level effects. Dose-, nanoform-, cell type-specific cytotoxicity changes were seen with both nanoSiO2 and nanoTiO2 exposures. Size, agglomeration, surface groups and metal impurities appeared to be cytotoxicity determinants. Proteomic data identified some common enriched mechanistic pathways relevant to phagocytosis, cell-shape changes, apoptosis, and inflammatory processes for nanoSiO2, nanoTiO2 exposed J774 cells. A549 cells exhibited pathway changes relevant to cell metabolism, endocytosis and inflammatory processes post-NP exposures. Concordance was seen between, cytotoxicity responses, notably cellular ATP, critical for cell viability, cellular oxidative stress and affected cellular pathways. These findings demonstrate the utility of proteomics in toxicology, warranting further exploration. [doi:10.25345/C5D50G848] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: NanoSiO2, NanoTiO2, In vitro toxicity, Nanoforms, Oxidative Stress, Proteomics, Cellular pathways

Contact

Principal Investigators:
(in alphabetical order) 		Dr. Premkumari Kumarathasan, Environmental Health Science and Research Bureau, HECSB, Health Canada, Ottawa, ON, Canada
Submitting User: srphanse
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