MassIVE MSV000086662

Complete Public PXD023421

The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry

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Description

Proteomic data from pulldown performed with the recombinant Campylobacter CiaD-GST fusion was expressed in E. coli BL21(DE3) on a lysate of human cultured INT 407 cells. One non-specific binding control was performed on INT 407 cell lysates and two-pulldowns were realized with the recombinant protein. Samples were digested with trypsin, then analyzed by LC-MS/MS. Data was searched with MS-GF+ using PNNL's DMS processing pipeline. [doi:10.25345/C5WV3B] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: proteomics ; pulldown ; CiaD ; campylobacter

Contact

Principal Investigators:
(in alphabetical order) 		Geremy Clair, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt

Publications

Negretti NM, Gourley CR, Talukdar PK, Clair G, Klappenbach CM, Lauritsen CJ, Adkins JN, Konkel ME.
The Campylobacter jejuni CiaD effector co-opts the host cell protein IQGAP1 to promote cell entry.
Nat Commun. 2021 Feb 26;12(1):1339. Epub 2021 Feb 26.

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