MassIVE MSV000082789

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MetaboLights MTBLS309 - GNPS "Metabolomic Responses of Guard Cells and Mesophyll Cells to Bicarbonate"

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Description

This data set was downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS309 Abstract:"Anthropogenic CO2 presently at 400 ppm is expected to reach 550 ppm in 2050, an increment expected to affect plant growth and productivity. Paired stomatal guard cells (GCs) are the gate-way for water, CO2, and pathogen, while mesophyll cells (MCs) represent the bulk cell-type of green leaves mainly for photosynthesis. We used the two different cell types, i.e., GCs and MCs from canola (Brassica napus) to profile metabolomic changes upon increased CO2 through supplementation with bicarbonate (HCO3-). Two metabolomics platforms enabled quantification of 268 metabolites in a time-course study to reveal short-term responses. The HCO3- responsive metabolomes of the cell types differed in their responsiveness. The MCs demonstrated increased amino acids, phenylpropanoids, redox metabolites, auxins and cytokinins, all of which were decreased in GCs in response to HCO3-. In addition, the GCs showed differential increases of primary C-metabolites, N-metabolites (e.g., purines and amino acids), and defense-responsive pathways (e.g., alkaloids, phenolics, and flavonoids) as compared to the MCs, indicating differential C/N homeostasis in the cell-types. The metabolomics results provide insights into plant responses and crop productivity under future climatic changes where elevated CO2 conditions are to take center-stage." [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Brassica napus

Contact

Principal Investigators:
(in alphabetical order) 		Chen, ufl.edu, us
Submitting User: rsilva
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