MassIVE MSV000082258

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Crocodile Sperm Proteome

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Description

In a reproductive strategy that is considered unique to the mammalian lineage, spermatozoa must undergo a series of physiological changes, termed capacitation, in the female reproductive tract prior to developing their capacity to fertilize an ovum. Here, we have employed a comparative proteomic strategy to explore the biological significance of this form of post-testicular maturation in the ancient reptilian species of the Australian saltwater crocodile (Crocodylus porosus). [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: Crocodylus porosu ; Proteome ; Phosphoproteome ; sperm

Contact

Principal Investigators:
(in alphabetical order) 		Brett Nixon, University of Newcastle, Australia
Dr. Matt Dun, University of Newcastle, Australia
Submitting User: Matt_MOG17

Publications

Nixon B, Johnston SD, Skerrett-Byrne DA, Anderson AL, Stanger SJ, Bromfield EG, Martin JH, Hansbro PM, Dun MD.
Proteomic profiling of crocodile spermatozoa refutes the tenet that post-testicular maturation is restricted to mammals.
Mol. Cell Proteomics. Epub 2018 Aug 2.

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Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

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Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

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Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
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