MassIVE MSV000096591

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Spatial proteomics of ER tubules reveals CLMN, an ER-actin tether at focal adhesions that promotes cell migration

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Description

Ctrl = control; R = replicate; en = endogenously tagged; mNG = mNeonGreen LUM1_1034603: Ctrl_U2OS_R1 LUM1_1034604: Ctrl_U2OS_R2 LUM1_1034605 Ctrl_U2OS_R3 LUM1_1034613 U2OS_RTN4en_TurboID_mNG_R1 LUM1_1034614 U2OS_RTN4en_TurboID_mNG_R2 LUM1_1034615 U2OS_RTN4en_TurboID_mNG_R3 LUM1_1034621 U2OS_mNG_TurboID_CLIMP63en_R1 LUM1_1034622 U2OS_mNG_TurboID_CLIMP63en_R2 LUM1_1034623 U2OS_mNG_TurboID_CLIMP63en_R3 LUM1_1034628 U2OS_CANXen_TurboID_mNG_R1 LUM1_1034629 U2OS_CANXen_TurboID_mNG_R2 LUM1_1034630 U2OS_CANXen_TurboID_mNG_R3 LUM1_1034632 U2OS_mNG_TurboID_LBRen_R1 LUM1_1034633 U2OS_mNG_TurboID_LBRen_R2 LUM1_1034634 U2OS_mNG_TurboID_LBRen_R3 LUM1_1034636 U2OS_mNG_TurboID_Nesprin4_R1 LUM1_1034637 U2OS_mNG_TurboID_Nesprin4_R2 LUM1_1034638 U2OS_mNG_TurboID_Nesprin4_R3 [doi:10.25345/C57D2QK59] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: spatial proteomics ; TurboID ; endoplasmic reticulum ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		W. Mike Henne, UT Southwestern, United States
Submitting User: alemoff
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Identification Results
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Quantification Results
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Number of distinct conditions across all analyses (original submission and reanalyses) associated with this dataset.

Distinct condition labels are counted across all files submitted in the "Metadata" category having a "Condition" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct biological replicates across all analyses (original submission and reanalyses) associated with this dataset.

Distinct replicate labels are counted across all files submitted in the "Metadata" category having a "BioReplicate" or "Replicate" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct technical replicates across all analyses (original submission and reanalyses) associated with this dataset.

The technical replicate count is defined as the maximum number of times any one distinct combination of condition and biological replicate was analyzed across all files submitted in the "Metadata" category. In the case of fractionated experiments, only the first fraction is considered.

"N/A" means no results of this type were submitted.
Originally identified proteins that were automatically remapped by MassIVE to proteins in the SwissProt human reference database.

"N/A" means no results of this type were submitted.
Number of distinct protein accessions reported across all analyses (original submission and reanalyses) associated with this dataset.

"N/A" means no results of this type were submitted.
Number of distinct unmodified peptide sequences reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct peptide sequences (including modified variants or peptidoforms) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Total number of peptide-spectrum matches (i.e. spectrum identifications) reported across all analyses (original submission and reanalyses) associated with this dataset.

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Number of distinct proteins quantified across all analyses (original submission and reanalyses) associated with this dataset.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
Number of distinct proteins found to be differentially abundant in at least one comparison across all analyses (original submission and reanalyses) associated with this dataset.

A protein is differentially abundant if its change in abundance across conditions is found to be statistically significant with an adjusted p-value <= 0.05 and lists no issues associated with statistical tests for differential abundance.

Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

"N/A" means no results of this type were submitted.
This dataset may not contain all raw spectra data as originally deposited in PRIDE. It has been imported to MassIVE for reanalysis purposes, so its spectra data here may consist solely of processed peak lists suitable for reanalysis with most software.