Serial Capture Affinity Purification (SCAP) samples analyzed using Multidimentional Protein identification Technology (MudPIT) on Orbitrap Velos Elite. Baits are SNAP-SPIN1 and Halo-WDR76. SNAP purification was performed first, then Halo purification was performed. SCAP was performed using whole cell extract from a stable cell line of 293FRT cells in WDR76 KO background. 3 biological replicates of E1, UB2 and E2 fractions.
[doi:10.25345/C5B79F]
[dataset license: CC0 1.0 Universal (CC0 1.0)]
Keywords: Halo tag ; SNAP tag ; AP-MS ; Serial Capture Affinity Purification ; MudPIT ; WDR76 ; SPIN1 ; SCAP-MS
Principal Investigators: (in alphabetical order) |
Michael P. Washburn, Stowers Institute for Medical Research, USA |
Submitting User: | xil_stowers |
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