MassIVE MSV000086167

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Comparative secretome analyses of toxigenic and atoxigenic Rathayibacter species

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Description

Phytopathogenic Rathayibacter species are unique bacterial plant pathogens as they are obligately vectored by plant parasitic Anguinid nematodes to the developing seedheads of forage grasses and cereals. This understudied group of plant associated Actinomycetes includes the neurotoxigenic Plant Pathogen Select Agent, R. toxicus, which causes annual ryegrass toxicity in grazing livestock. The complexRathayibacter disease cycle requires intimate interactions with the nematode cuticle and plant hosts, which warrants an increased understanding of the secretory and surface-associated proteins that mediate these diverse eukaryotic interactions. Here we present the first comparative secretome analysis for this complex, nematode vectoredRathayibacter genera that compares three agronomically damaging Rathayibacterspecies, R. toxicus, R. iranicus, and R. tritici. The exoproteomic comparison identified 1,423 unique proteins between the three species using LC-MS/MS. Of the uniquely identified proteins, 94 homologous proteins were conserved between the three Rathayibacter exoproteomes and comprised between 43.4 to 58.6% of total protein abundance. Comparative analyses revealed both conserved and uniquely expressed extracellular proteins, which, interestingly, had more similarities to extracellular proteins commonly associated with bacterial animal pathogens than classical plant pathogens. This comparative exoproteome analysis will facilitate the characterization of proteins essential for vector and host colonization and assist in the development of diagnostic targets. [doi:10.25345/C57B4N] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: rathayibacter secretomes

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Principal Investigators:
(in alphabetical order) 		Matthew A Tancos, Foreign Disease Weed Science Research Unit, United States Department of Agriculture Agricultural Research Service, Frederick, MD, United States
Submitting User: mbmcmahon
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Distinct protein accessions are counted across all files submitted in the "Statistical Analysis of Quantified Analytes" category having a "Protein" column in this dataset.

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