MassIVE MSV000100300

Complete Public PXD072270

Proteomic analysis of phosphorylation in HCoV-229E-infected immortalized human lung fibroblasts (MRC5)

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Description

TMT18-labeled proteome-wide profiling of post-translational modifications (phosphorylation) in mock-infected and infected human lung epithelial cells (MRC5). Human lung epithelial (MRC5) cells were infected and mock-infected with HCoV-229E. Cells were harvested at 8, 16, and 24 hours post-infection. Extracted proteins were digested with trypsin, then TMT18-labeled and enriched for phosphorylation. Samples were run on the Q Exactive HF-X. Data was searched with MS-GF+ using PNNL's DMS Processing pipeline. [doi:10.25345/C5RF5KV3D] [dataset license: CC0 1.0 Universal (CC0 1.0)]

Keywords: HCoV-229E ; coronavirus ; human lung ; post-translational modifications ; redox ; DatasetType:Proteomics

Contact

Principal Investigators:
(in alphabetical order) 		Amy Sims, Pacific Northwest National Laboratory, United States
Tong Zhang, Pacific Northwest National Laboratory, United States
Submitting User: alchemistmatt
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